Date of Award
6-1-2015
Document Type
Masters Thesis
Degree Name
M.S.
Organizational Unit
College of Natual Science and Mathematics
First Advisor
Michelle K. Knowles, Ph.D.
Second Advisor
Bryan J. Cowen
Third Advisor
Brian J. Majestic
Fourth Advisor
Mark E. Siemens
Keywords
Biosensor, Gold nanoparticle, Lipid oxidation, Lipoxygenase, LSPR
Abstract
Gold nanoparticles have been intensively studied for their unique optical features. Due to surface plasmon resonance phenomenon, gold nanoparticles can respond to the refractive index change of the environment near the particles. This phenomenon gives gold nanoparticles the potential to become biosensors that detect the biological interactions at or near the particles. In this work, gold nanospheres were coated with phosphatidylcholine as the substrate for lipoxygenase. It is demonstrated, in our work, that lipid coated gold nanospheres can be used to detect the activity of lipoxygenase and provide more information of this reaction than common assays, like conjugated diene assay and TBARS assay. After demonstrating that lipoxygenase activity can be measured with gold nanoparticles, asymmetric gold nanoprisms were synthesized. The purpose of this was to create a more sensitive sensor for future studies of lipid-protein interactions. The long-term goal of the work is to create a versatile biological sensor that can detect enzyme activities under different environments.
Publication Statement
Copyright is held by the author. User is responsible for all copyright compliance.
Rights Holder
Yuheng Cai
Provenance
Received from ProQuest
File Format
application/pdf
Language
en
File Size
62 p.
Recommended Citation
Cai, Yuheng, "Lipid-Coated Gold Nanoparticle as a Biosensor for Lipid-Protein Interactions" (2015). Electronic Theses and Dissertations. 105.
https://digitalcommons.du.edu/etd/105
Copyright date
2015
Discipline
Biophysics