Imaging Disulfide Dinitroxides at 250 MHz to Monitor Thiol Redox Status

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Disulfide dinitroxide, Disulfide cleavage, Glutathione, Image reconstruction, Rapid-scan EPR

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College of Natual Science and Mathematics, Chemistry and Biochemistry, Daniel Felix Ritchie School of Engineering and Computer Science


Measurement of thiol–disulfide redox status is crucial for characterization of tumor physiology. The electron paramagnetic resonance (EPR) spectra of disulfide-linked dinitroxides are readily distinguished from those of the corresponding monoradicals that are formed by cleavage of the disulfide linkage by free thiols. EPR spectra can thus be used to monitor the rate of cleavage and the thiol redox status. EPR spectra of 1H,14N- and 2H,15N-disulfide dinitroxides and the corresponding monoradicals resulting from cleavage by glutathione have been characterized at 250 MHz, 1.04 GHz, and 9 GHz and imaged by rapid-scan EPR at 250 MHz.

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