Imaging Disulfide Dinitroxides at 250 MHz to Monitor Thiol Redox Status

Authors

Hanan B. Elajaili, University of DenverFollow
Joshua R. Biller, University of DenverFollow
Gerald M. Rosen, University of Maryland - Baltimore
Joseph P. Y. Kao, University of Maryland - Baltimore
Mark Tseitlin, University of Denver
Laura A. Buchanan, University of DenverFollow
George A. Rinard, University of DenverFollow
Richard W. Quine, University of DenverFollow
Joseph McPeak, University of Denver
Yilin Shi, University of Denver
Sandra S. Eaton, University of DenverFollow
Gareth R. Eaton, University of DenverFollow

Publication Date

11-2015

Document Type

Article

Organizational Units

College of Natual Science and Mathematics, Chemistry and Biochemistry, Daniel Felix Ritchie School of Engineering and Computer Science

Keywords

Disulfide dinitroxide, Disulfide cleavage, Glutathione, Image reconstruction, Rapid-scan EPR

Abstract

Measurement of thiol–disulfide redox status is crucial for characterization of tumor physiology. The electron paramagnetic resonance (EPR) spectra of disulfide-linked dinitroxides are readily distinguished from those of the corresponding monoradicals that are formed by cleavage of the disulfide linkage by free thiols. EPR spectra can thus be used to monitor the rate of cleavage and the thiol redox status. EPR spectra of 1H,14N- and 2H,15N-disulfide dinitroxides and the corresponding monoradicals resulting from cleavage by glutathione have been characterized at 250 MHz, 1.04 GHz, and 9 GHz and imaged by rapid-scan EPR at 250 MHz.

Publication Statement

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Recommended Citation

Elajaili, H., Biller, J. R., Rosen, G. M., Kao, J. P. Y., Tseytlin, M., Buchanan, L. A., . . . Eaton, G. R. (2015). Imaging disulfide dinitroxides at 250 MHz to monitor thiol redox status. Journal of Magnetic Resonance (1997), 260, 77-82. DOI: 10.1016/j.jmr.2015.08.027.