Date of Award
Schuyler Van Engelenburg
During angiogenesis, the development of new blood vessels from existing vessels, there must be tight regulation between a leading tip cell and trailing stalk cells during vascular patterning. Lateral inhibition produced by Notch signaling is central to the specification and maintenance of tip and stalk cells in growing angiogenic sprouts. The Notch pathway is activated by the ligand Delta at adherens junctions between tip and stalk cells. Despite the centrality of Delta/Notch signaling to angiogenesis, relatively little is known about the mechanism of this signaling event. It has been proposed that the force generated by the Notch bound Delta ligand exposes a cleavage site necessary for Notch activation. Here, we show for the first time in endothelial cells that the proteins Epsin homology domain protein 2 (EHD2) and EHD2 binding partner 1 (EHBP1) regulate Delta like ligand 4 (Dll4) endocytosis by tethering its caveolar endocytic pit to the actin cytoskeleton.
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Webb, Amelia Margaret, "EHBP1 and EHD2 Regulate Dll4 Caveolin-Mediated Endocytosis During Blood Vessel Development" (2020). Electronic Theses and Dissertations. 1860.
Received from ProQuest
Amelia Margaret Webb
Cellular biology, Biology, Molecular biology