Date of Award
J. Todd Blankenship
EHD2, Endothelial cells, Biophysics
The role of EHD2 in terms of Cav1 interaction had not been previously characterized and was the main goal of this research. Cav1 has been shown to lead to the ubiquitination of Rac1. Because of this, the role of EHD2 in Rac1 regulation was investigated. An increase in long-term migration in endothelial cells was observed and suggested that EHD2 impacts the RhoA pathway instead of the Rac1 pathway. This lead to the hypothesis that EHD2 controls a part of the RhoA pathway. This hypothesis is supported by the loss of filamentous actin and an increase in serrated junctions in cells that have an excess of EHD2. This change is not just seen in cells - when EHD2 is knocked out in fish, there are visible changes in the junctions that are similar to what occurs in the EHD2si Endothelial cells.
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Judson, Rachael, "The Role of EHD2 in Endothelial Cells" (2022). Electronic Theses and Dissertations. 2137.
Received from ProQuest