Date of Award
J. Todd Blankenship
Michelle K. Knowles
Cells, Extracellular vesicles, Microvesicles, Ectocytosis
Extracellular vesicles (EVs) are membrane derived organelles released by all cell types and carry a vast array of cargos that can influence communication, development, and disease. One type of EV, known as microvesicles, form by budding directly from the plasma membrane in a process known as ectocytosis, however the mechanisms that govern this process are poorly understood. In C. elegans, the P4-ATPase, TAT-5, along with its activator, PAD-1, inhibit EV release from the plasma membrane by inhibiting phosphatidylethanolamine (PE) exposure on the outer leaflet of the plasma membrane. We identified 3 key domains of PAD-1 that are required to inhibit EV release and characterized alleles of TAT-5 and PAD-1, including a PAD-1 gain-of-function allele. These newly identified domains and characterized alleles provide us new opportunities to test the in vivo roles of TAT-5 and PAD-1, which in return will help us better understand the mechanisms that govern EV biogenesis.
Copyright is held by the author. User is responsible for all copyright compliance.
Pitts, Lauren, "Extracellular vesicles and lipid flippase regulators in C. elegans" (2022). Electronic Theses and Dissertations. 2155.
Received from ProQuest
Available for download on Thursday, September 26, 2024