Date of Award
James T. Blankenship, Ph.D.
Drosophila, Convergent extension, F-actin
Convergent extension is a process that occurs in the development of a wide variety of organisms, including gastrulation in the Drosophila embryo to begin to lay out the adult body plan. In fly embryos, this is known as germband extension and is mainly driven by cell intercalation or neighbor exchange by planar polarized cell-cell interface contraction to shorten the tissue along the dorsal-ventral axis. In this thesis, I show that interface contraction consists of phases of fast interface shortening and intervals of stable interface size. My data also suggests that regulation of F-actin aggregates at these shrinking interfaces is important for appropriate biphasic interface contraction. Knock down of two F-actin regulating proteins, Dpod1 and coronin, results in aberrant interface dynamics and severe disruption of germband extension. Close examination of interface and F-actin aggregate dynamics in coronin knock down reveals that coronin is required for appropriate F-actin globule formation and interface contraction.
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Motlong, Ashley M., "Regulation of Actin Dynamics During Drosophila Germband Extension" (2013). Electronic Theses and Dissertations. 457.
Received from ProQuest
Developmental biology, Cellular biology