Date of Award

1-1-2013

Document Type

Masters Thesis

Degree Name

M.S.

Organizational Unit

Biological Sciences

First Advisor

J. T. Blankenship, Ph.D.

Second Advisor

Joseph Angleson

Third Advisor

Scott Barbee

Fourth Advisor

Darrin Hicks

Keywords

Gastrulation, Cell intercalation, Dynamin, Endocytosis

Abstract

Gastrulation, a process conserved among many higher organisms, is the directed migration of cells into layers that will establish various tissues targeted to become anatomical structures. This process is accomplished through another conserved morphogenetic event, known as cell intercalation. Early in development, this movement of cells within an organized tissue leads to unique cellular arrangements where neighboring cells contract their shared interfaces in order to meet at a shared vertex. In this thesis, I present work that demonstrates a requirement for Dynamin-dependent endocytosis during these contraction events. Using quantitative analysis, I have identified varied cell behaviors during experiments which knockdown the function of dynamin. In addition, I demonstrate the existence of an antagonistic relationship between Dynamin and the Myosin II motor protein. Lastly, localization and functional studies I performed for this work suggest a role for Sorting Nexin proteins during plasma membrane reorganization required for Dynamin-dependent endocytosis.

Publication Statement

Copyright is held by the author. User is responsible for all copyright compliance.

Rights Holder

Marissa Kay Kuhl

Provenance

Received from ProQuest

File Format

application/pdf

Language

en

File Size

103 p.

Discipline

Developmental Biology

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