Publication Date
11-22-2019
Document Type
Article
Organizational Units
College of Natural Science and Mathematics, Biological Sciences
Keywords
Human immunodeficiency virus 1 (HIV-1), Single-particle tracking, Virus assembly
Abstract
The HIV-1 envelope glycoprotein (Env) is sparsely incorporated onto assembling virus particles on the host cell plasma membrane in order for the virus to balance infectivity and evade the immune response. Env becomes trapped in a nascent particle on encounter with the polymeric viral protein Gag, which forms a dense protein lattice on the inner leaflet of the plasma membrane. While Env incorporation efficiency is readily measured biochemically from released particles, very little is known about the spatiotemporal dynamics of Env trapping events. Herein, we demonstrate, via high-resolution single-molecule tracking, that retention of Env trimers within single virus assembly sites requires the Env cytoplasmic tail (CT) and the L12 residue in the matrix (MA) domain of Gag but does not require curvature of the viral lattice. We further demonstrate that Env trimers are confined to subviral regions of a budding Gag lattice, supporting a model where direct interactions and/or steric corralling between the Env-CT and a lattice of MA trimers promote Env trapping and infectious HIV-1 assembly.
Copyright Date
11-22-2019
Copyright Statement / License for Reuse
This work is licensed under a Creative Commons Attribution 4.0 International License.
Rights Holder
The Authors
Provenance
Received from author
File Format
application/pdf
Language
English (eng)
Extent
9 pgs
File Size
2055 KB
Publication Statement
Copyright is held by the authors. User is responsible for all copyright compliance. This article was originally published as:
Pezeshkian, N., Groves, N. S., & van Engelenburg, S. B. (2019). Single-molecule imaging of HIV-1 envelope glycoprotein dynamics and Gag lattice association exposes determinants responsible for virus incorporation. Proceedings of the National Academy of Sciences - PNAS, 116(50), 25269–25277. DOI: 10.1073/pnas.1910008116
Publication Title
Proceedings of the National Academy of Sciences
Volume
116
Issue
50
First Page
25269
Last Page
25277
ISSN
1091-6490
Recommended Citation
Pezeshkian, N., Groves, N. S., & van Engelenburg, S. B. (2019). Single-molecule imaging of HIV-1 envelope glycoprotein dynamics and Gag lattice association exposes determinants responsible for virus incorporation. Proceedings of the National Academy of Sciences - PNAS, 116(50), 25269–25277. DOI: 10.1073/pnas.1910008116