Date of Award
Chemistry and Biochemistry
Martin Margittai, Ph.D.
Alzheimer's disease, Progressive Supranuclear Palsy, Protein aggregation, Tau, Tauopathy
The protein Tau is found in neurofibrillary tangles in Alzheimer's disease and over 20 other neurodegenerative diseases. An assay has been developed to detect minute amounts of fibrils from human brain tissue. This assay subjects brain tissue extract and recombinant Tau to several rounds of sonication and incubation. Incubation allows recombinant Tau to add itself to the ends of the existing fibrils in brain tissue extract. Sonication breaks the existing fibrils in the brain tissue extract offering more ends for Tau to add onto. Cycles of sonication and incubation have been shown to allow for amplification of Tau fibrils from Alzheimer's disease tissue. This assay can be used not only for detection, but also to note differences between tauopathies and to look at various agents that have been shown to block fibril growth.
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Hager, Emily Rickman, "Improving the Detection Limit of Tau Aggregates for Use with Biological Samples" (2018). Electronic Theses and Dissertations. 1417.
Received from ProQuest
Emily Rickman Hager
Biochemistry Commons, Biostatistics Commons, Other Biochemistry, Biophysics, and Structural Biology Commons