Date of Award
Robert M. Dores, Ph.D.
MC2R, MC5R, MRAP1, Rainbow Trout
Using CHO cells we sought to explore and characterize the functional relationship of rainbow trout MC5 receptor (rtMC5R) with zebrafish MRAP1 (zfMRAP1) and rainbow trout MC2 receptor (rtMC2R), as well as how the effect of such relationships may play a significant role in Hypothalamic-Pituitary-Adrenal/Interrenal axis activation. This research demonstrated that rtMC5R can be successfully expressed and functionally activated in CHO cells and, in a manner similar to mammals, α-MSH is the preferred ligand for rtMC5R. The presence of MRAP1 does not seem to inhibit the expression or function of rtMC5R, rather it appears that it may increase expression and total activation levels. In contrast, the presence of MRAP1 in conjunction with MC2R reduces expression and total activation levels of rtMC5R. Findings indicate that rtMC5R may function in three different ways in a cell, depending upon the presence of other local proteins. The interaction of rtMC5R with MRAP1 and MC2R, and the implications of such interactions will be described in this thesis.
Hill, Domicinda M., "Analyzing the Interaction between Melanocortin 5 Receptor, Melanocortin Receptor Accessory Protein 1, and Melanocortin 2 Receptor in Rainbow Trout" (2012). Electronic Theses and Dissertations. 288.
Received from ProQuest
Domicinda M. Hill