Date of Award

6-1-2012

Document Type

Masters Thesis

Degree Name

M.S.

Organizational Unit

College of Natual Science and Mathematics

First Advisor

Robert M. Dores, Ph.D.

Second Advisor

Daniel Linseman

Third Advisor

Nancy Lorenzon

Fourth Advisor

Scott Pegan

Keywords

MC2R, MC5R, MRAP1, Rainbow Trout

Abstract

Using CHO cells we sought to explore and characterize the functional relationship of rainbow trout MC5 receptor (rtMC5R) with zebrafish MRAP1 (zfMRAP1) and rainbow trout MC2 receptor (rtMC2R), as well as how the effect of such relationships may play a significant role in Hypothalamic-Pituitary-Adrenal/Interrenal axis activation. This research demonstrated that rtMC5R can be successfully expressed and functionally activated in CHO cells and, in a manner similar to mammals, α-MSH is the preferred ligand for rtMC5R. The presence of MRAP1 does not seem to inhibit the expression or function of rtMC5R, rather it appears that it may increase expression and total activation levels. In contrast, the presence of MRAP1 in conjunction with MC2R reduces expression and total activation levels of rtMC5R. Findings indicate that rtMC5R may function in three different ways in a cell, depending upon the presence of other local proteins. The interaction of rtMC5R with MRAP1 and MC2R, and the implications of such interactions will be described in this thesis.

Publication Statement

Copyright is held by the author. User is responsible for all copyright compliance.

Rights Holder

Domicinda M. Hill

Provenance

Received from ProQuest

File Format

application/pdf

Language

en

File Size

56 p.

Discipline

Endocrinology, Biology

Download



Share

COinS