Date of Award
College of Natual Science and Mathematics
Thomas W. Quinn, Ph.D.
Transposable elements, TE, Genomic evolution, Transposition mechanism
Transposable elements (TE) have been found in all genomes and have clearly had a major impact on genomic evolution. The described research takes advantage of an abundant transposable element present in the genomes of Anseriformes, called Chicken Repeat 1 (CR1). Previous studies in Anseriformes suggest that CR1 is presently active in recent evolutionary time (St. John, 2004). A fully functional CR1 element itself is approximately 4.5kb long (Kajikawa, 1997), where almost all inserts are truncated at the 5' end. Because of this, it has been a challenge to isolate a full length, active element. In this study, two CR1 sequences were obtained after screening a genomic library of Cape Barren Goose using probes complimentary to the flanking regions of the element. The findings unveiled sequences with a complete ORF1, ORF2, 3' untranslated region and a portion of the 5' untranslated region. This study gets one step closer a further understanding the transposition mechanism that are adopted by this class of TE's, a non-long terminal repeat (non-LTR) retransposons. Eventually, the capture of an active element could make it especially valuable for future research by investigating their ability to transpose in living cells.
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Cassandra Michelle Weason
Received from ProQuest
Weason, Cassandra Michelle, "Isolation and Characterization of a Full Length Retrotransposon: CR1" (2013). Electronic Theses and Dissertations. 691.