Date of Award
Joseph Angleson, Ph.D.
Robert M. Dores
Alpha cells, cAMP, Cyclic AMP, Endocrinology, Epac, PKA, Protein kinase A
Blood glucose levels are regulated by the interactions between insulin and glucagon. Pancreatic alpha cells release glucagon in response to a drop in blood sugar. Pancreatic alpha cells appear to be regulated through multiple signaling pathways. One potential method of regulation occurs from α-MSH or epinephrine stimulated production of the intracellular messenger cyclic AMP. Exocytosis of dense core vesicles containing glucagon ultimately requires an increase in cytosolic calcium. This study focused on the link between cyclic AMP and calcium in pancreatic alpha cells which allows alpha cell regulation. Interactions between calcium and cyclic AMP in pancreatic alpha cells consists of PKA-dependant and PKA-independent pathways. The results indicate cyclic AMP pathways control L-type voltage-gated calcium channels and also increase cytoplasmic calcium from intracellular stores. Store release appears to be through IP3 mediated pathways and not through the ryanodine-sensitive receptor.
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Imseis, Julianne Michelle, "Assessing Crosstalk Between Calcium and cAMP in Pancreatic Alpha Cells" (2009). Electronic Theses and Dissertations. 829.
Received from ProQuest
Julianne Michelle Imseis
Cellular biology, Molecular biology