Title

Nanoscale Coupling of Endocytic Pit Growth and Stability

Document Type

Article

Publication Date

11-27-2019

Compass Link

https://du-primo.hosted.exlibrisgroup.com/permalink/f/1jii0mc/TN_cdi_proquest_miscellaneous_2322743548

Organizational Units

College of Natual Science and Mathematics, Physics and Astronomy

Abstract

Clathrin-mediated endocytosis, an essential process for plasma membrane homeostasis and cell signaling, is characterized by stunning heterogeneity in the size and lifetime of clathrin-coated endocytic pits (CCPs). If and how CCP growth and lifetime are coupled and how this relates to their physiological function are unknown. We combine computational modeling, automated tracking of CCP dynamics, electron microscopy, and functional rescue experiments to demonstrate that CCP growth and lifetime are closely correlated and mechanistically linked by the early-acting endocytic F-BAR protein FCHo2. FCHo2 assembles at the rim of CCPs to control CCP growth and lifetime by coupling the invagination of early endocytic intermediates to clathrin lattice assembly. Our data suggest a mechanism for the nanoscale control of CCP growth and stability that may similarly apply to other metastable structures in cells.

Publication Statement

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