Publication Date
9-11-2017
Document Type
Article
Organizational Units
College of Natural Science and Mathematics, Biological Sciences
Keywords
Transcriptome, Morphological, Phylogenetical, Homoscleromorph sponge, Mitochondrial genome
Abstract
The homoscleromorph sponge Oscarella carmela, first described from central California, USA is shown to represent two superficially similar but both morphologically and phylogenetically distinct species that are co-distributed. We here describe a new species as Oscarella pearsei, sp. nov. and re-describe Oscarella carmela; the original description was based upon material from both species. Further, we correct the identification of published genomic/transcriptomic resources that were originally attributed to O. carmela, and present new Illumina-sequenced transcriptome assemblies for each of these species, and the mitochondrial genome sequence for O. pearsei sp. nov. Using SSU and LSU ribosomal DNA and the mitochondrial genome, we report the phylogenetic relationships of these species relative to other Oscarella species, and find strong support for the placement of O. pearsei sp. nov. in a distinct clade within genus Oscarella defined by the presence of spherulous cells that contain paracrystalline inclusions; O. carmela lacks this cell type. Oscarella pearsei sp. nov and O. carmela can be tentatively distinguished based upon gross morphological differences such as color, surface texture and extent of mucus production, but can be more reliably identified using mitochondrial and nuclear barcode sequencing, ultrastructural characteristics of cells in the mesohyl, and the morphology of the follicle epithelium which surrounds the developing embryo in reproductively active individuals.
Copyright Date
9-11-2017
Copyright Statement / License for Reuse
This work is licensed under a Creative Commons Attribution 4.0 International License.
Rights Holder
The Authors
Provenance
Received from author
File Format
application/pdf
Language
English (eng)
Extent
25 pgs
File Size
44608 KB
Publication Statement
Copyright is held by the authors. User is responsible for all copyright compliance. This article was originally published as:
Ereskovsky, A. V. , Richter, D. J., Lavrov, D. V., Schippers, K. J., & Nichols, S. A. (2017). Transcriptome sequencing and delimitation of sympatric Oscarella species (O. carmela and O. pearsei sp. nov) from California, USA. PloS One, 12(9), 1-25. DOI: 10.1371/journal.pone.0183002
Publication Title
PLOS One
Volume
12
Issue
9
First Page
1
Last Page
25
ISSN
1932-6203
Recommended Citation
Ereskovsky, A. V. , Richter, D. J., Lavrov, D. V., Schippers, K. J., & Nichols, S. A. (2017). Transcriptome sequencing and delimitation of sympatric Oscarella species (O. carmela and O. pearsei sp. nov) from California, USA. PloS One, 12(9), 1-25. DOI: 10.1371/journal.pone.0183002