Publication Date
7-9-2018
Document Type
Article
Organizational Units
College of Natural Science and Mathematics, Biological Sciences, Physics and Astronomy
Keywords
Cell intercalation, Tricellular vertices, Oscillatory cycles, Asymmetric radial force
Abstract
Oriented cell intercalation is an essential developmental process that shapes tissue morphologies through the directional insertion of cells between their neighbors. Previous research has focused on properties of cell–cell interfaces, while the function of tricellular vertices has remained unaddressed. Here, we identify a highly novel mechanism in which vertices demonstrate independent sliding behaviors along cell peripheries to produce the topological deformations responsible for intercalation. Through systematic analysis, we find that the motion of vertices connected by contracting interfaces is not physically coupled, but instead possess strong radial coupling. E-cadherin and Myosin II exist in previously unstudied populations at cell vertices and undergo oscillatory cycles of accumulation and dispersion that are coordinated with changes in cell area. Additionally, peak enrichment of vertex E-cadherin/Myosin II coincides with interface length stabilization. Our results suggest a model in which asymmetric radial force balance directs the progressive, ratcheted motion of individual vertices to drive intercalation.
Copyright Date
7-9-2018
Copyright Statement / License for Reuse
This work is licensed under a Creative Commons Attribution 4.0 International License.
Rights Holder
The Authors
Provenance
Received from author
File Format
application/pdf
Language
English (eng)
Extent
23 pgs
File Size
5558 KB
Publication Statement
Copyright held by the authors. User is responsible for all copyright compliance. This article was originally published as:
Vanderleest, T. E., Smits, C. M., Xie, Y., Jewett, C. E., Blankenship, J. T., & Loerke, D. (2018). Vertex sliding drives intercalation by radial coupling of adhesion and actomyosin networks during Drosophila germband extension. eLife, 7, 1-23. DOI: 10.7554/elife.34586
Publication Title
eLife
Volume
7
First Page
1
Last Page
23
ISSN
2050-084X
Recommended Citation
Vanderleest, T. E., Smits, C. M., Xie, Y., Jewett, C. E., Blankenship, J. T., & Loerke, D. (2018). Vertex sliding drives intercalation by radial coupling of adhesion and actomyosin networks during Drosophila germband extension. eLife, 7, 1-23. DOI: 10.7554/elife.34586