Effects of Overexpression and Inhibited Expression of Thymosin, an Actin‐Interacting Protein from Bombyx Mori, on BmNPV Proliferation and Replication

Authors

• Jinxu Liao, Institute of Biochemistry, Zhejiang Sci‐Tech University, Zhejiang Provincial Key Laboratory of Silkworm Bioreactor and Biomedicine
• Chen Zhang, University of Denver
• Wenjuan Ru, University of Texas Medical Branch
• Dan Wang, Institute of Biochemistry, Zhejiang Sci‐Tech University, Zhejiang Provincial Key Laboratory of Silkworm Bioreactor and Biomedicine
• Wenping Zhang, Institute of Biochemistry, Zhejiang Sci‐Tech University, Zhejiang Provincial Key Laboratory of Silkworm Bioreactor and Biomedicine

Publication Date

1-29-2018

Document Type

Article

Organizational Units

College of Natural Science and Mathematics, Biological Sciences

Keywords

Actin, Bombyx mori, Bombyx mori nucleopolyhedrovirus, Thymosin

Abstract

Previous study showed that exogenously applied recombinant thymosin from Bombyx mori (BmTHY) reduces B. mori nucleopolyhedrovirus (BmNPV) proliferation in silkworm. Which stands to reason that BmTHY in B. mori is crucial for the defense against BmNPV. However, little is known about the effect of endogenously overexpressed or repressed BmTHY on B. mori resistance to virus infection. To study this issue, we constructed an overexpression and inhibited expression systems of BmTHY in BmN cells. The viral titer and the analysis from the quantitative real‐time polymerase chain reaction (PCR) revealed that overexpression of BmTHY decreased the copies of BmNPV gene gp41, which goes over to inhibit the proliferation of BmNPV in BmN cells, while the inhibited expression of BmTHY significantly enhanced viral proliferation in infected BmN cells. These results indicated that endogenous BmTHY can inhibit BmNPV proliferation and replication in infected BmN cells. Furthermore, Co‐IP showed that BmTHY could bind to actin in BmN cells. Also, the overexpression or inhibited expression of BmTHY shifted the ratio of F/G‐actin in infected BmN cells. Lastly, the BmTHY, an actin‐interacting protein, might be one of the key host factors against BmNPV, which inhibits viral proliferation and replication in BmN cells.

Copyright Date

1-29-2018

Copyright Statement / License for Reuse

All Rights Reserved.

Rights Holder

Wiley Periodicals, Inc.

Provenance

Received from author

Language

English (eng)

Extent

10 pgs

Publication Statement

Copyright is held by Wiley Periodicals, Inc. User is responsible for all copyright compliance.

Publication Title

Archives of Insect Biochemistry and Physiology

Volume

98

Issue

1

First Page

1

Last Page

10

ISSN

1520-6327

Recommended Citation

Liao, J., Zhang, C., Ru, W., Wang, D., & Zhang, W. (2018). Effects of overexpression and inhibited expression of thymosin, an actin‐interacting protein from Bombyx mori, on BmNPV proliferation and replication. Archives of Insect Biochemistry and Physiology, 98(1), 1-10. DOI: 10.1002/arch.21449

DOI Link

https://doi.org/10.1002/arch.21449