Publication Date
9-26-2023
Document Type
Article
Organizational Units
College of Natural Science and Mathematics, Biological Sciences
Keywords
Large dense core vesicles, HID-1, Endocrine system, Neuropeptides, Peptides
Abstract
Large dense core vesicles (LDCVs) mediate the regulated release of neuropeptides and peptide hormones. HID-1 is a trans-Golgi network (TGN) localized peripheral membrane protein contributing to LDCV formation. There is no information about HID-1 structure or domain architecture, and thus it remains unknown how HID-1 binds to the TGN and performs its function. We report that the N-terminus of HID-1 mediates membrane binding through a myristoyl group with a polybasic amino acid patch but lacks specificity for the TGN. In addition, we show that the C-terminus serves as the functional domain. Indeed, this isolated domain, when tethered to the TGN, can rescue the neuroendocrine secretion and sorting defects observed in HID-1 KO cells. Finally, we report that a point mutation within that domain, identified in patients with endocrine and neurological deficits, leads to loss of function.
Copyright Date
9-26-2023
Copyright Statement / License for Reuse
This work is licensed under a Creative Commons Attribution 4.0 International License.
Rights Holder
Blake H. Hummer, Theodore Carter, Breanna L. Sellers, Jenna D. Triplett, and Cedric S. Asensio
Provenance
Received from Faculty Publications Booklet
File Format
application/pdf
Language
English (eng)
Extent
14 pgs
File Size
1.75 MB
Publication Statement
Copyright is held by the authors. User is responsible for all copyright compliance. This article was originally published as:
Hummer, B. H., Carter, T., Sellers, B. L., Triplett, J. D., & Asensio, C. S. (2023). Identification of the functional domain of the dense core vesicle biogenesis factor HID-1. PLOS ONE, 18(9), e0291977. https://doi.org/10.1371/journal.pone.0291977
Publication Title
PLoS ONE
Volume
18
Issue
9
First Page
e0291977
ISSN
1932-6203
PubMed ID
37751424
Recommended Citation
Hummer, Blake H.; Carter, Theodore; Sellers, Breanna L.; Triplett, Jenna D.; and Asensio, Cedric S., "Identification of the Functional Domain of the Dense Core Vesicle Biogenesis Factor HID-1" (2023). Biological Sciences: Faculty Scholarship. 68.
https://digitalcommons.du.edu/biological_sciences_faculty/68
https://doi.org/10.1371/journal.pone.0291977