Publication Date
9-26-2023
Document Type
Article
Organizational Units
College of Natural Science and Mathematics, Biological Sciences
Keywords
Large dense core vesicles, HID-1, Endocrine system, Neuropeptides, Peptides
Abstract
Large dense core vesicles (LDCVs) mediate the regulated release of neuropeptides and peptide hormones. HID-1 is a trans-Golgi network (TGN) localized peripheral membrane protein contributing to LDCV formation. There is no information about HID-1 structure or domain architecture, and thus it remains unknown how HID-1 binds to the TGN and performs its function. We report that the N-terminus of HID-1 mediates membrane binding through a myristoyl group with a polybasic amino acid patch but lacks specificity for the TGN. In addition, we show that the C-terminus serves as the functional domain. Indeed, this isolated domain, when tethered to the TGN, can rescue the neuroendocrine secretion and sorting defects observed in HID-1 KO cells. Finally, we report that a point mutation within that domain, identified in patients with endocrine and neurological deficits, leads to loss of function.
Copyright Date
9-26-2023
Copyright Statement / License for Reuse
This work is licensed under a Creative Commons Attribution 4.0 International License.
Publication Statement
Copyright is held by the authors. User is responsible for all copyright compliance. This article was originally published as:
Hummer, B. H., Carter, T., Sellers, B. L., Triplett, J. D., & Asensio, C. S. (2023). Identification of the functional domain of the dense core vesicle biogenesis factor HID-1. PLOS ONE, 18(9), e0291977. https://doi.org/10.1371/journal.pone.0291977
Rights Holder
Blake H. Hummer, Theodore Carter, Breanna L. Sellers, Jenna D. Triplett, and Cedric S. Asensio
Provenance
Received from Faculty Publications Booklet
File Format
application/pdf
Language
English (eng)
Extent
14 pgs
File Size
1.75 MB
Publication Title
PLoS ONE
Volume
18
Issue
9
First Page
e0291977
ISSN
1932-6203
PubMed ID
37751424
Recommended Citation
Hummer, Blake H.; Carter, Theodore; Sellers, Breanna L.; Triplett, Jenna D.; and Asensio, Cedric S., "Identification of the Functional Domain of the Dense Core Vesicle Biogenesis Factor HID-1" (2023). Biological Sciences: Faculty Scholarship. 68.
https://digitalcommons.du.edu/biological_sciences_faculty/68
https://doi.org/10.1371/journal.pone.0291977