Date of Award
1-1-2016
Document Type
Masters Thesis
Degree Name
M.S.
Organizational Unit
Daniel Felix Ritchie School of Engineering and Computer Science, Center for Orthopaedic Biomechanics
First Advisor
Ali N. Azadani, Ph.D.
Second Advisor
James Fogleman
Third Advisor
Matthew Gordon
Fourth Advisor
Breigh Roszelle
Keywords
Cardiac biomechanics, Crystalloid, Ex-vivo, Langendorff, Porcine, Slaughterhouse
Abstract
The objective of this study was to resuscitate isolated beating porcine hearts obtained from slaughterhouse swine for a minimum of 1 hour with a crystalloid buffer reperfusion instead of blood via an extracorporeal Langendorff apparatus. The isolated beating heart functions outside of the body under simulated physiologic conditions. Live functional anatomy was controlled under benchtop experimental settings. Porcine hearts (26 total) were topically cooled with saline. 300mL of 25°C cardioplegia with 20KU Streptokinase was preflushed via aortic root. WIT was less than 5 minutes. 1L 4°C cardioplegia was flushed antegrade with aortic cannula (Group 1) or coronary catheterization (Group 2). Each heart was kept in 4°C 1L modified Plegisol storage for >1 hour cold ischemic time prior to resuscitation. Hearts were submerged in 500mL of 15°C and 25°C Krebs Henseleit buffer prior to being mounted on the Langendorff apparatus. Hearts were resuscitated by 5L of 37°C oxygenated modified Krebs Hensleit buffer circulation. Low WIT, Streptokinase, and coronary catheterization resulted in 1 hour global contraction, and 110bpm stabilized sinus rhythm. ECG displayed prominent QRS complex. Significant calcium overload and edema inhibited contractile function at 1 hr. The Langendorff beating heart apparatus will serve as a platform for further biomedical engineering research to evaluate the efficacy of medical devices such as heart valves and validate computational simulations.
Publication Statement
Copyright is held by the author. User is responsible for all copyright compliance.
Rights Holder
Rahiemin Talukder
Provenance
Received from ProQuest
File Format
application/pdf
Language
en
File Size
153 p.
Recommended Citation
Talukder, Rahiemin, "Ex-Vivo Slaughterhouse Porcine Crystalloid-Perfused Beating Heart via Langendorff Method" (2016). Electronic Theses and Dissertations. 1102.
https://digitalcommons.du.edu/etd/1102
Copyright date
2016
Discipline
Biomedical Engineering, Biology