Date of Award

1-1-2017

Document Type

Masters Thesis

Degree Name

M.S.

Organizational Unit

Biological Sciences

First Advisor

Joseph K. Angleson, Ph.D.

Second Advisor

Cedric Asensio

Third Advisor

Schuyler van Engelenburg

Fourth Advisor

Eric Boschmann

Keywords

Anterior pituitary, Exocytosis, IA2, Islet antibody 2, Lactotrophs, Phogrin, Synaptotagmin

Abstract

Peptides, which are packaged in dense core vesicles, are an integral part of the function of the endocrine and neurological systems. The dense core vesicles function as an efficient form of peptide storage prior to regulated exocytosis. Two different dense core specific transmembrane proteins traffic different when comparted to retained prolactin cores, offering evidence of heterogeneity of vesicles within a single cell. By comparing synaptotagmin 1 and 7 distribution in male rat and lactating female lactotrophs, a distinct pattern emerges. Cells that retain prolactin cores after exocytosis correspond with those that contain synaptotagmin 1. This finding is a reversal for previous studies in chromaffin cells supporting the theory that not all dense core vesicles are the same across cell types. These experiments offer evidence of two different levels of heterogeneity in dense core vesicles across cell types and within a single cell. This variation allows cells to have robust regulation over secretion, an necessity in these vital systems.

Publication Statement

Copyright is held by the author. User is responsible for all copyright compliance.

Rights Holder

Kelly Sinak

Provenance

Received from ProQuest

File Format

application/pdf

Language

en

File Size

45 p.

Discipline

Cellular biology, Molecular biology, Biology

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