Date of Award
Schuyler B. Van Engelenburg, Ph.D.
HIV-1, HIV type 1, HIV, Env, Envelope glycoprotein, iPALM, Interferometric PALM, Superresolution, Virus assembly
Human Immunodeficiency Virus type 1 (HIV-1) replicates by forcing infected host cells to produce new virus particles, which assemble form protein components on the inner leaflet of the host cell's plasma membrane. This involves incorporation of the essential viral envelope glycoprotein (Env) into a structural lattice of viral Gag proteins. The mechanism of Env recruitment and incorporation is not well understood. To better define this process, we seek to describe the timing of Env-Gag encounters during particle assembly by measuring angular positions of Env proteins about the surfaces of budding particles. Using three-dimensional superresolution microscopy, we show that Env distributions are biased toward the necks of budding particles, indicating incorporation of Env late in the assembly of the lattice. We show that this behavior is dependent on the host cell type and on the long cytoplasmic tail of Env. We propose a model wherein Env incorporation is regulated by opposing mechanisms: Gag lattice trapping of Env cytoplasmic tails, and intracellular sequestering of Env during lattice assembly.
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Carmen Anne Buttler
Received from ProQuest
Buttler, Carmen Anne, "Superresolved Three-Dimensional Analysis of the Spatial Arrangement of the Human Immunodeficiency Virus Type-1 (HIV-1) Envelope Glycoprotein at Sites of Viral Assembly" (2018). Electronic Theses and Dissertations. 1449.
Virology, Molecular biology, Cellular biology