Date of Award
2022
Document Type
Masters Thesis
Degree Name
M.S.
Organizational Unit
College of Natural Science and Mathematics, Biological Sciences
First Advisor
Ann Wehman
Second Advisor
J. Todd Blankenship
Third Advisor
Karen Krukowski
Keywords
C. elegans, Corpse clearance, Non-apoptotic, Phosphatidylserine, PS externalization, Scramblase
Abstract
Cell corpse clearance is key for animals to avoid inflammation and autoimmune disease. To better understand the “eat me” signal of phosphatidylserine (PS) exposure for non-apoptotic corpse clearance, we attempted to disrupt the externalization of PS on C. elegans polar bodies. The second polar body undergoes a non-apoptotic form of cell death, loses membrane integrity, externalizes PS and is cleared by embryonic cells during early development. During apoptotic and necrotic cell death, PS asymmetry is disrupted by lipid scramblases, which translocate phospholipids between the leaflets of the lipid bilayer. We found neither apoptotic nor necrotic scramblases are required for PS externalization on dying polar bodies. These results lead us closer to defining the role of PS in phagocytic uptake as well as later steps of corpse membrane breakdown. However, which scramblases regulate PS exposure on non-apoptotic dying cells is still unknown.
Publication Statement
Copyright is held by the author. User is responsible for all copyright compliance.
Rights Holder
Julia Frondoni
Provenance
Received from ProQuest
File Format
application/pdf
Language
en
File Size
75 pgs
Recommended Citation
Frondoni, Julia, "Phosphatidylserine Externalization on Non-Apoptotic Cells and Lipid Scramblases in C. Elegans" (2022). Electronic Theses and Dissertations. 2051.
https://digitalcommons.du.edu/etd/2051
Copyright date
2022
Discipline
Molecular biology, Cellular biology