Date of Award
J. Todd Blankenship
C. elegans, Corpse clearance, Non-apoptotic, Phosphatidylserine, PS externalization, Scramblase
Cell corpse clearance is key for animals to avoid inflammation and autoimmune disease. To better understand the “eat me” signal of phosphatidylserine (PS) exposure for non-apoptotic corpse clearance, we attempted to disrupt the externalization of PS on C. elegans polar bodies. The second polar body undergoes a non-apoptotic form of cell death, loses membrane integrity, externalizes PS and is cleared by embryonic cells during early development. During apoptotic and necrotic cell death, PS asymmetry is disrupted by lipid scramblases, which translocate phospholipids between the leaflets of the lipid bilayer. We found neither apoptotic nor necrotic scramblases are required for PS externalization on dying polar bodies. These results lead us closer to defining the role of PS in phagocytic uptake as well as later steps of corpse membrane breakdown. However, which scramblases regulate PS exposure on non-apoptotic dying cells is still unknown.
Copyright is held by the author. User is responsible for all copyright compliance.
Frondoni, Julia, "Phosphatidylserine Externalization on Non-Apoptotic Cells and Lipid Scramblases in C. Elegans" (2022). Electronic Theses and Dissertations. 2051.
Received from ProQuest
Molecular biology, Cellular biology
Available for download on Friday, July 21, 2023