Date of Award

2022

Document Type

Masters Thesis

Degree Name

M.S.

Organizational Unit

College of Natural Science and Mathematics, Biological Sciences

First Advisor

Ann Wehman

Second Advisor

Joe Angleson

Third Advisor

J. Todd Blankenship

Fourth Advisor

Michelle K. Knowles

Keywords

Cells, Extracellular vesicles, Microvesicles, Ectocytosis

Abstract

Extracellular vesicles (EVs) are membrane derived organelles released by all cell types and carry a vast array of cargos that can influence communication, development, and disease. One type of EV, known as microvesicles, form by budding directly from the plasma membrane in a process known as ectocytosis, however the mechanisms that govern this process are poorly understood. In C. elegans, the P4-ATPase, TAT-5, along with its activator, PAD-1, inhibit EV release from the plasma membrane by inhibiting phosphatidylethanolamine (PE) exposure on the outer leaflet of the plasma membrane. We identified 3 key domains of PAD-1 that are required to inhibit EV release and characterized alleles of TAT-5 and PAD-1, including a PAD-1 gain-of-function allele. These newly identified domains and characterized alleles provide us new opportunities to test the in vivo roles of TAT-5 and PAD-1, which in return will help us better understand the mechanisms that govern EV biogenesis.

Publication Statement

Copyright is held by the author. User is responsible for all copyright compliance.

Rights Holder

Lauren Pitts

Provenance

Received from ProQuest

File Format

application/pdf

Language

en

File Size

92 pgs

Discipline

Biology

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