"Unraveling the Role of HID-1: Insights into SgII Regulation" by Breanna L. Sellers

Date of Award

Summer 8-24-2024

Document Type

Masters Thesis

Degree Name

M.S. in Biological Sciences

Organizational Unit

College of Natural Science and Mathematics, Biological Sciences

First Advisor

Cedric Asensio

Second Advisor

J. Todd Blankenship

Third Advisor

Ann M. Wehman

Copyright Statement / License for Reuse

All Rights Reserved
All Rights Reserved.

Keywords

HID-1, Large dense core vesicles (LDCV), Regulated secretory pathway

Abstract

The regulated secretory pathway is important for the secretion of hormones and neuropeptides from large dense core vesicles (LDCVs). Dysregulation of the biogenesis of LDCVs can result in metabolic and neurological diseases. Recent studies have suggested that the peripheral membrane protein HID-1 plays a role in the sorting and storage of granins which contribute to LDCV biogenesis. To further understand how HID-1 influences the regulation of granins, we investigated potential HID-1 interactors. We determined that while HID-1 is likely functioning in the same pathway as USP25, a deubiquitinating enzyme (DUB), the cause of reduced cellular SgII levels is likely not due to increased degradation. We also characterized the role of phosphorylation within HID-1’s disordered region and found that it didn’t affect HID-1’s function or localization. Further investigation into HID-1 will allow us to unravel the mysteries behind SgII regulation, which may lead to pharmaceutical targets for metabolic and neurological diseases.

Copyright Date

8-2024

Publication Statement

Copyright is held by the author. User is responsible for all copyright compliance.

Rights Holder

Breanna L. Sellers

Provenance

Received from Author

File Format

application/pdf

Language

English (eng)

Extent

51 pgs

File Size

6.4 MB

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