Date of Award
Spring 6-12-2026
Document Type
Masters Thesis
Degree Name
M.S. in Biological Sciences
Organizational Unit
College of Natural Science and Mathematics, Biological Sciences
First Advisor
Mira Pronobis
Second Advisor
Yan Qin
Third Advisor
Erich Kushner
Copyright Statement / License for Reuse
All Rights Reserved.
Keywords
Proximity-labeling, Split-TurboID, TurboID, Zebrafish, Heart disease
Abstract
Heart disease is the leading cause of death globally. Unlike mammals, which often develop scarring due to limited heart regeneration, zebrafish can completely regenerate their hearts. Studies have shown that the endocardium and epicardium secrete key mitogens that enhance regeneration. However, only a few of these mitogens have been identified in zebrafish. To capture changes in signaling networks between the myocardium and the epicardium or endocardium, we are deploying an in vivo proximity-labeling method by using split-TurboID in adult zebrafish hearts. This technique involves dividing the full-length TurboID biotin enzyme into two inactive fragments that reassemble during protein-protein interactions or membrane-membrane contact. In this thesis, we show successful generation of 3 transgenic split-TurboID zebrafish lines. This approach will enable labeling and identification of specific protein networks at these interfaces during heart regeneration.
Copyright Date
6-2026
Publication Statement
Copyright is held by the author. User is responsible for all copyright compliance.
Rights Holder
Chuol Wal Kueth
Provenance
Received from ProQuest
File Format
application/pdf
Language
English (eng)
Extent
29 pgs
File Size
841 KB
Recommended Citation
Kueth, Chuol Wal, "In Vivo Proximity Labeling Techniques to Capture Proteomic Changes at the Cardiomyocyte-Endothelial and Epicardial Interface" (2026). Electronic Theses and Dissertations. 2727.
https://digitalcommons.du.edu/etd/2727