Date of Award
1-1-2012
Document Type
Masters Thesis
Degree Name
M.S.
Organizational Unit
College of Natual Science and Mathematics
First Advisor
John C. Kinnamon, Ph.D.
Second Advisor
Dwight Smith
Third Advisor
Scott Barbee
Fourth Advisor
Nancy Lorenzon
Keywords
Chemoreception, Immunohistochemistry, Norepinephrine, Taste
Abstract
Of the handful of neurotransmitters modulating taste response, norepinephrine (NE) may be the least understood. Although the physiological role of NE has been tested, it is still unclear whether the rat taste bud may serve as an endogenous source of neurotransmitter or if it must be taken up from outside the taste bud. Immunocytochemical analysis of DBH has shown that DBH–LIR is present in taste cells of the rat circumvallate papillae. Specifically, DBH–LIR is present in a subset of Type II taste cells. Nearly all (98%) DBH–LIR cells express PLCβ2–LIR, and about half (41%) of PLCβ2–LIR cells express DBH–LIR. DBH–LIR does not co–localize with NCAM, and thus, is not present in Type III cells.
NET–LIR is present in the taste cells of the rat circumvallate papillae. Double labeling of NET–LIR and IP 3R3–LIR indicates that NET is present in Type II taste cells. A large proportion (77%) of NET–LIR cells express IP3R3–LIR and all (100%) IP3R3–LIR cells express NET–LIR. A small proportion (7.8%) of NET–LIR cells also express syntaxin–1–LIR in Type III cells. About a third (32.8%) of syntaxin–1–LIR cells express NET–LIR.
Publication Statement
Copyright is held by the author. User is responsible for all copyright compliance.
Rights Holder
Jenna Ashley Walton
Provenance
Received from ProQuest
File Format
application/pdf
Language
en
File Size
88 p.
Recommended Citation
Walton, Jenna Ashley, "Dopamine-Beta-Hydroxylase and Norepinephrine Transporter Immunoreactivity in Rat Taste Buds" (2012). Electronic Theses and Dissertations. 684.
https://digitalcommons.du.edu/etd/684
Copyright date
2012
Discipline
Biology, Neurosciences