Date of Award

1-1-2010

Document Type

Dissertation

Degree Name

Ph.D.

First Advisor

Andrei G. Kutateladze, Ph.D.

Second Advisor

Joseph Angleson, Ph.D.

Third Advisor

Sandra S. Eaton

Fourth Advisor

Joseph Hornback

Fifth Advisor

Michelle Knowles

Keywords

Assays, Fluorescence, Molecular recognition, Photoamplification

Abstract

During the course of this research a novel method which couples the molecular recognition-triggered photoamplification chain in diaryl ketone adducts of dithiane with a "turn-off" or "turn-on" fluorescence-based assay for the detection of biological targets and ligands, regardless of their nature, through a molecular recognition event has been developed. This research has included several key steps, the most significant being: (1) the design of fluorophore adducts or dyads which recover fluorescence upon photocleavage for a "turn-on" assay and the identification of fluorophores which are quenched upon the photochemical release of a quencher for a "turn off" assay; (2) Optimization of the photochemistry and photoamplification of dithiane adducts of benzophenone as it applies to the development of an ultra-sensitive photoamplified fluorescence assay; (3) implementation of the method in the design and fabrication of chips for ultra-sensitive screening of microarrays, and (4) integration of this microchip assay into a fluorescence based signal transducer for ultra-sensitive detection of molecular recognition events. Proof of concept utilized biotin-avidin recognition, where avidin is coupled to the photochemical sensitizer.

Publication Statement

Copyright is held by the author. User is responsible for all copyright compliance.

Rights Holder

Tiffany Priscilla Gustafson

Provenance

Received from ProQuest

File Format

application/pdf

Language

en

File Size

231 p.

Discipline

Organic chemistry

Download



Share

COinS