Date of Award
8-1-2013
Document Type
Masters Thesis
Degree Name
M.S.
Organizational Unit
College of Natual Science and Mathematics
First Advisor
Joseph K. Angleson, Ph.D.
Second Advisor
David Patterson
Third Advisor
Nancy Lorenzon
Fourth Advisor
Robert Amme
Keywords
Ca2+ /calmodulin-dependent protein kinase II (CaMKII), GABAA-receptors, α-cells
Abstract
Calcium/calmodulin-dependent protein kinase II (CaMKII) has been identified as an important modulator in controlling electrical activity in neurons and the heart; however, a role for CaMKII in pancreatic alpha cell signaling has not been previously reported. Upon activation by calcium/calmodulin, CaMKII phosphorylates proteins involved in intracellular calcium homeostasis by inducing downstream effects such as an increase in AMPA-receptor single channel conductance, potentiation of L-type voltage dependent calcium channels, and enhanced surface expression of inhibitory GABAA-receptors. In the pancreas, an increase in intracellular calcium drives secretion of glucagon from alpha cells within the Islets of Langerhans. α-cells contain many of the known targets of CaMKII described in neurons, including AMPA/GluR1 receptors, L-type VGCC and GABAA-receptors. CaMKII may also regulate other aspects of glucagon secretion, aside from calcium homeostasis, such as vesicle trafficking, exocytosis, and cytoskeleton dynamics. Based on the expression of CaMKII targets and the requirement of calcium influx for hormone secretion, we explored which CaMKII genes, if any, are found in α-cells, and are involved in the regulation of α-cell signaling and glucagon release.
Publication Statement
Copyright is held by the author. User is responsible for all copyright compliance.
Rights Holder
Brooke A. Buckland
Provenance
Received from ProQuest
File Format
application/pdf
Language
en
File Size
76 p.
Recommended Citation
Buckland, Brooke A., "Role of CaMKII in Pancreatic Alpha Cells" (2013). Electronic Theses and Dissertations. 96.
https://digitalcommons.du.edu/etd/96
Copyright date
2013
Discipline
Cellular biology