Date of Award
Spring 6-15-2024
Document Type
Undergraduate Honors Thesis
Degree Name
B.S in Molecular Biology
Organizational Unit
Biological Sciences, College of Natural Science and Mathematics
First Advisor
Stanley Kanai
Second Advisor
David Clouthier
Third Advisor
Nancy Lorenzon
Keywords
Single-cell RNA sequencing, Cranial neural crest cells (CNCC), Early B-cell factor 3 (ebf3a), Pharyngeal arches, Sanger sequencing, Gel electrophoresis, Colorimetric in situ hybridization (ISH), Fluorescence, Sense probe, Antisense probe, Plasmid, Restriction digest, Embryonic development, Expression pattern, Cell cycle regulator, Pluripotent stem cells (PSCs)
Abstract
Proper craniofacial development requires many genes. Single-cell RNA sequencing (scRNA-seq) allows us to identify genes expressed in precursor cells for craniofacial structures, but the function of many of these genes in craniofacial development has yet to be characterized. In our scRNA-seq of cranial neural crest cells (NCCs), which are precursor cells for the craniofacial skeleton, we found a gene called early B-cell factor 3 (ebf3a) that may be involved in craniofacial development. In our scRNA-seq data, ebf3a expression is restricted to cranial NCCs of the dorsal and ventral domains of pharyngeal arches 1 and 2. In humans, damaging variants in EBF3 are associated with facial dysmorphism. Based on these observations, we hypothesize that ebf3a is an important gene in craniofacial development. In this study, we begin to test this hypothesis by characterizing the expression pattern of ebf3a in cranial NCCs in zebrafish. Through fluorescence in situ hybridization, we confirmed that ebf3a is expressed in the cranial NCCs of the dorsal and ventral patterning domains of pharyngeal arches 1 and 2. This result will be a starting point for future experiments that will investigate the function of ebf3a in craniofacial development.
Copyright Date
2-22-2024
Copyright Statement / License for Reuse
This work is licensed under a Creative Commons Attribution 4.0 International License.
Publication Statement
Copyright is held by the author. User is responsible for all copyright compliance.
Rights Holder
Shujan A. Sharafeldeen
Provenance
Received from author
File Format
application/pdf
Language
English (eng)
Extent
19 pgs
File Size
546 KB
Recommended Citation
Sharafeldeen, Shujan A., "Investigating the Role of ebf3a in Craniofacial Development" (2024). Undergraduate Theses, Capstones, and Recitals. 33.
https://digitalcommons.du.edu/undergraduate_theses/33
Included in
Oral Biology and Oral Pathology Commons, Other Dentistry Commons, Other Genetics and Genomics Commons