Date of Award

2020

Document Type

Masters Thesis

Degree Name

M. S.

Organizational Unit

College of Natual Science and Mathematics, Biological Sciences

First Advisor

Erich Kushner

Second Advisor

Martin Margittai

Third Advisor

Schuyler Van Engelenburg

Fourth Advisor

Cedric Asensio

Keywords

Angiogenesis

Abstract

During angiogenesis, the development of new blood vessels from existing vessels, there must be tight regulation between a leading tip cell and trailing stalk cells during vascular patterning. Lateral inhibition produced by Notch signaling is central to the specification and maintenance of tip and stalk cells in growing angiogenic sprouts. The Notch pathway is activated by the ligand Delta at adherens junctions between tip and stalk cells. Despite the centrality of Delta/Notch signaling to angiogenesis, relatively little is known about the mechanism of this signaling event. It has been proposed that the force generated by the Notch bound Delta ligand exposes a cleavage site necessary for Notch activation. Here, we show for the first time in endothelial cells that the proteins Epsin homology domain protein 2 (EHD2) and EHD2 binding partner 1 (EHBP1) regulate Delta like ligand 4 (Dll4) endocytosis by tethering its caveolar endocytic pit to the actin cytoskeleton.

Publication Statement

Copyright is held by the author. User is responsible for all copyright compliance.

Rights Holder

Amelia Margaret Webb

Provenance

Received from ProQuest

File Format

application/pdf

Language

en

File Size

69 p.

Discipline

Cellular biology, Biology, Molecular biology

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