Date of Award
8-2023
Document Type
Masters Thesis
Degree Name
M.S.
Organizational Unit
College of Natural Science and Mathematics, Biological Sciences
First Advisor
Ann Wehman
Second Advisor
J. Todd Blankenship
Third Advisor
Cedric Asensio
Keywords
ATG-16, Cell corpse, LC3-associated phagocytosis, Macroautophagy, Membrane breakdown, Polar body
Abstract
Failure to digest cell corpses can lead to lupus-like autoimmune disorders in mammals. To better understand if LC3-associated phagocytosis (LAP) drives cell corpse breakdown in phagolysosomes, we studied the clearance of the C. elegans polar body during embryonic development. ATG16L1 has separable roles in regulating LC3 recruitment during macroautophagy and LAP. We demonstrated that the ATG16L1 C. elegans orthologs, ATG-16.1 and ATG-16.2, function redundantly to promote polar body membrane breakdown. We also discovered that truncating the LAP-specific WD40 domain of ATG-16.2 unexpectedly disrupts autophagy. Furthermore, we confirmed that polar body membrane breakdown occurs independent of macroautophagy. We also showed that LC3 localizes to the phagolysosome independent of autophagosomes. Although these findings show macroautophagy is unlikely to be promoting polar body degradation, the mechanism by which LC3 is recruited to the polar body phagolysosome to promote cell corpse breakdown remains unknown.
Copyright Date
8-2023
Copyright Statement / License for Reuse
All Rights Reserved.
Publication Statement
Copyright is held by the author. User is responsible for all copyright compliance.
Rights Holder
Shruti Kolli
Provenance
Received from ProQuest
File Format
application/pdf
Language
English (eng)
Extent
81 pgs
File Size
3.6 MB
Recommended Citation
Kolli, Shruti, "Polar Body Membrane Breakdown Occurs Independent of Macroautophagy in C. Elegans" (2023). Electronic Theses and Dissertations. 2294.
https://digitalcommons.du.edu/etd/2294
Discipline
Molecular biology, Cellular biology, Biology